Grouping of oriental tobacco of Krumovgrad and Basma (Greek) ecotypes from different regions and micro regions of Haskovo and Djebel tobacco growing area by important chemical indicators
Violeta Nikolova, Radka Bozhinova, Nikolay Nikolov, Yovko Dyulgerski
Abstract: In a study conducted in 2018, the chemical composition (nicotine, reducing sugars, nicotine/reducing sugars ratio, total nitrogen, ash and potassium) of oriental tobacco of two ecotypes from different regions and micro regions of Haskovo and Djebel growing area was investigated. Hierarchical cluster analysis was used to organize the tobacco with similar values of the measured chemical indicators into groups. The samples of Krumovgrad ecotype from the different micro regions of Haskovo and Djebel production area are grouped into three clusters. A separate cluster is formed by the samples from Strandzhevo (Krumovgrad region), Djebel (Djebel region), Patitsa (Kardzhali region), Stambolovo (Haskovo-Harmanli region), Buk (Krumovgrad region) and Krumovgrad (Krumovgrad region), which have a more balanced chemical composition. The main indicators that have the greatest influence on the division of tobaccos of Krumovgrad ecotype from the different microregions into clusters are the nicotine/reducing sugars ratio and the nicotine, reducing sugars, total nitrogen and ash content, united in the first factor, explaining 67.49% of the total variance of the variables. Regarding the chemical composition of tobacco of Basma (Greek) ecotype, from different micro regions of the Haskovo production area, three clusters are also formed. The first of them includes samples from the micro regions Chorbadzhiysko (Momchilgrad region) and Buk (Krumovgrad region), which have a higher nicotine content and a lower reducing sugars/nicotine ratio, and in this case it is an indication of a better balance in smoking properties. A significant proportion (55.32%) of the variance was explained by first factor, which correlates strongly with nicotine, total nitrogen and ash content, as well as the reducing sugars/nicotine ratio. The results obtained can be used in evaluating the quality of tobaccos as a raw material for smoking products, in view of their optimal usability in blending.
Keywords: chemical composition; cluster analysis; grouping; micro regions; oriental tobacco; principal component analysis
Citation: Nikolova, V., Bozhinova, R., Nikolov, N., & Dyulgerski, Y. (2025). Grouping of oriental tobacco of Krumovgrad and Basma (Greek) ecotypes from different regions and micro regions of Haskovo and Djebel tobacco growing area by important chemical indicators. Bulgarian Journal of Soil Science Agrochemisty and Ecology, 59(2), 3-15 (Bg).
References: (click to open/close) | Atanasov, D., & Nestorov, A. (1981). Tobacco production and curing. Hristo G. Danov, Plovdiv (Bg). Banožić, M., Jokić, S., Ačkar, Đ., Blažić, M., & Šubarić, D. (2020). Carbohydrates - Key players in tobacco aroma formation and quality determination. Molecules, 25(7), 1734. BDS 15836:1988. Tobacco and tobacco products. Methods of total nitrogen determination (Bg). Castano, J. I., Vargas, L. R., & Palacio, F. J. (1990). Evaluation of tobacco grading systems by multivariate analysis of their chemical quality parameters. In: CORESTA Congress. Symposium, Oct (pp. 1-2). https://www.coresta.org/abstracts/evaluation-tobacco-grading-systems-multivariate-analysis-their-chemical-quality-0 (last accessed 14.12.2024). Fang, W. Kaichao, L., Jin, H., Xiansheng, Z., Yunbai, W., Yang, N., Qiang, M., & Zhanying, Z. (2009). Cluster Analysis on Relationship between Tobacco Growth Regionand Chemical Compositions of Flue-cured Tobacco Leaves. Chinese Tobacco Science, 30(2), 57-61. Ganeva, Z. (2016). Discovering statistics using IBM SPSS statistics. Elestra, Sofia (Bg). Gyuzelev, L. (1983). Stick science of tobacco. Hristo G. Danov, Plovdiv (Bg). Gilchrist, S. N. (1999). Field practices. In: Tobacco Production, Chemistry and Technology (Davis D., Nielsen M., eds.). Blackwell Science, Cambridge, UK, 154-163. ISO 15152:2003. Tobacco - Determination of the content of total alkaloids as nicotine - Continuous - flow analysis method. ISO 15154:2003. Tobacco - Determination of the content of reducing carbohydrates - Continuous - flow analysis method. ISO 2817:1999. Tobacco and tobacco products - Determination of silicate residues in soluble in hydrochloric acid. Kınay, A., & Kurt, D. (2021). Chemical content and quality of sun cured tobacco lines. Anadolu Tarım Bilimleri Dergisi, 36(2), 282-292. Kurt, D. (2021). Impacts of environmental variations on quality and chemical contents of oriental tobacco. Contributions to Tobacco & Nicotine Research, 30(1), 50-62. Kurt, D., Kınay, A., Saygılı, İ., & Kandemir, N. (2022). Determining the genetic and agronomic variations in lines from Samsun tobacco growing areas. Anadolu Tarım Bilimleri Dergisi, 37(3), 617-636. Ma, J. M., Liu, G. S., Shi, X. D., Wei, Y. W., Lu, X. M., Li, Y. J., & Ye, X. F. (2009). Application of principal component analysis and cluster analysis in tobacco quality evaluation. Tobacco Science and Technology, 7, 57-60. Şahin, O., & Ekren, S. (2022). Identification of high nicotine oriental tobacco lines. MAS Journal of Applied Sciences, 7(4), 959-974. Sekin, S., Peksuslu, A., & Küçüközden, R. (2002). Macro and micro element contents of Izmir tobaccos related with quality. In: The Second Balkan Scientific Conference on “Quality and efficiency of the tobacco production, treatment and processing”, 47-55. Tang, Z., Chen, L., Chen, Z., Fu, Y., Sun, X., Wang, B., & Xia, T. (2020). Climatic factors determine the yield and quality of Honghe flue-cured tobacco. Scientific Reports, 10, 19868. Tanov, E., & Milyanchev, I. (1974). Natural conditions for the development of tobacco production in the Republic of Bulgaria. In: Tobacco in Bulgaria (Donev N., Zlatev G., eds.). Hristo G. Danov, Plovdiv, 23-62 (Bg). Tepecik, M., & Ongun, A. (2020). Determination of some quality parameters of oriental type tobacco based on harvest times. Turkish Journal of Agricultural Research, 7(2), 156-162. Tsaliki, E., Moysiadis, T., Toumpas, E., Kalivas, A., Panoras, I., & Grigoriadis, I. (2023). Evaluation of Greek Tobacco Varieties (Nicotiana tabacum L.) Grown in Different Regions οf Greece. Agriculture, 13(7), 1394. Tso, T. C. (1990). Production, Physiology and Biochemistry of Tobacco Plant. Ideals, Inc., Beltsville, Maryland. Volodarskiy, N. I. (1971). Mineral Nutrition of Tobacco. In: Physiology of Agricultural Plants (Rubin B. A., ed.). Moskow University, Moskow, 196-243 (Ru). Ward, J. H. (1963). Hierarchical Grouping to Optimize an Objective Function. Journal of the American Statistical Association, 58, 236-244. Yancheva, D., Dagnon, S., & Stoilova, A. (2008). Mineral fertilization diagnostics of oriental tobacco variety Krumovgrad. Varietal response. Plant Science, 45(4), 343-346 (Bg).
|
|
| Date published: 2025-06-25
Download full text