Micronutrient concentration in garden pea genotypes as affected by inoculation of arbuscular mycorrhizal fungi and plant growth promoting rhizobacteria
Slavka Kalapchieva, Radka Bozhinova, Tsveta Hristeva
Abstract: The influence of a microbial inoculant Europlus®, containing AM fungi (Glomus sp.) and beneficial rhizobacteria (Bacillus sp.), on the concentration of micronutrients (Fe, Mn, Zn and Cu) in the above-ground biomass and roots of six garden pea genotypes was studied. The experiment was conducted under field conditions in the Maritsa Vegetable Crop Research Institute on Alluvial-meadow soil.
It was found that the microbial inoculant had a weak effect on the agrochemical properties of the soil. The combined inoculation increased the concentration of Fe in the aboveground biomass of most genotypes by 2.4%-22.9%. The increase of iron in the roots was by 4.4%-12.6%. Application of combined inoculant, in some cases, increased the Mn concentration in the aboveground biomass of genotypes - by 9.3%-35.7% compared to the controls. This was associated with an increased in Zn concentrations (+2.1%-64.9%) and Cu concentrations (+5.2%-20.2%) in aboveground biomass of inoculated genotypes. Microbial inoculant application increased root Mn, Zn and Cu concentrations by 4.8%-35.1%, 5.3%-20.8% and 3.3%-24.4%, respectively, compared to non-inoculated controls.
The response of the individual pea genotypes to the formation of symbiosis with AM fungi was different. The bioinoculant increased root colonization rates with AM fungi by over 7% on average. A correlation was recorded only with Fe concentrations.
The genotype factor had a greater effect on the micronutrient concentrations in pea samples. Inoculation with AM fungi and beneficial rhizobacteria had a weaker effect, but it was also statistically significant in terms of the concentration of Fe, Mn and Zn in aboveground biomass and roots.
Hierarchical cluster analysis classified the aboveground samples of pea genotypes from the inoculated and non-inoculated treatments into two main clusters based on the concentration of micronutrients. The 12 root samples were divided into 3 groups.
Keywords: Bacillus; garden pea genotypes; Glomus; microbial inoculation; micronutrients
Citation: Kalapchieva, S., Bozhinova, R., & Hristeva, T. (2024). Micronutrient concentration in garden pea genotypes as affected by inoculation of arbuscular mycorrhizal fungi and plant growth promoting rhizobacteria. Bulgarian Journal of Soil Science Agrochemisty and Ecology, 58(4), 21- 34 (Bg).
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| Date published: 2024-12-19
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