Investigation of the effect of anolyte for surface disinfection

Toshka Petrova, Teodora Popova

Abstract: The effect of an anolyte prepared by electrolysis of water with 0.3% NaCl for disinfection of surfaces after 10 min of exposure compared to that of 1% chlorhexidine was studied. With sterile swabs, wash samples were taken from the surface of laboratory workbenches and from the laboratory sink trough (10 cm2 area) after 10 minutes of exposure to the antimicrobials used, applied by spraying the surface. For control, wash samples were taken without treatment with detergent. The sample materials were cultured on Müeller-Hinton agar for 24 h under aerobic conditions at 37°C. Species and quantitative testing of bacteria was performed using paper disks for detection of pathogenic bacteria HiDtectTM - Rapid Identification Discs (Universal Enviro for detection of E. coli, S. aureus, P. aeruginosa, E. faecalis, S. Typhimurium and UTI for E. coli, S. aureus, P. aeruginosa, E. faecalis, K. pneumoniae, P. mirabilis) as prescribed by the manufacturer. The results obtained show a very similar antimicrobial effect of the two tested agents, and that the anolyte can be used for disinfection of such surfaces with the same success as the broad-spectrum disinfectant chlorhexidine.

Keywords: anolyte; chlorhexidine; disinfection; electrochemically activated water; washes samples

Date published: 2022-08-23